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TRC-00204 | CD40 Ligand (Human) ELISA Kit | 96 Well

Product Overview

A sandwich ELISA kit for the quantitative measurement of CD40 Ligand in Human serum, plasma, tissue homogenates and other biological fluids.

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. 96Trace’s CD40 Ligand ELISA kit is a sandwich ELISA assay for measuring the quantity of human CD40 Ligand in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with fewer incubation and wash steps respectively, resulting in less errors and more consistent results.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityHuman
Sample TypeHuman serum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range45 - 4000 pg/ml
Sensitivity< 14 pg/ml
Assay Time90 minutes

Applications

This ELISA kit is used for quantitative measurement of CD40 Ligand in Human serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Designed for quantitative measurement of CD40 Ligand.
  • Assay format: Sandwich ELISA.
  • Sample type: Human serum, plasma, tissue homogenates and other biological fluids.
  • Detection method: Absorbance (450 nm).
  • Detection Range: 45 - 4000 pg/ml
  • Sensitivity: < 14 pg/ml
  • Assay Time: 90 minutes.
  • Kit size: 96 Well.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

Catalog NumberTRC-00204
Analyte / TargetCD40 Ligand