TRC-00172 | Caspase-3 (CASP3) (Rat) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the quantitative measurement of Caspase-3 in rat serum, plasma, culture supernatants and other biological fluids.
Caspase-3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. 96Trace’s CASP3 ELISA kit is a sandwich ELISA assay for the quantitative measurement of CASP3 in Rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of CASP3 captured from the samples.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Rat |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.313 – 20 ng/ml |
| Sensitivity | < 0.188 ng/ml |
Applications
This ELISA kit is used for quantitative measurement of caspase-3 in rat serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits
- Easy, convenient and time-saving method to measure the level of caspase-3 in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
- Detection Range: 0.313 – 20 ng/ml
- Sensitivity: < 0.188 ng/ml
- Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
- No significant cross-reactivity or interference was observed.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4592-100 |
| Supplier CAT | TRC-00172 |
| Analyte / Target | Caspase-3 (CASP3) |
| Category | Cancer Biomarker ELISA Kits |