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TRC-00172 | Caspase-3 (CASP3) (Rat) ELISA Kit | 96 Well

Product Overview

A Sandwich ELISA kit for the quantitative measurement of Caspase-3 in rat serum, plasma, culture supernatants and other biological fluids.

Caspase-3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. 96Trace’s CASP3 ELISA kit is a sandwich ELISA assay for the quantitative measurement of CASP3 in Rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of CASP3 captured from the samples.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityRat
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range0.313 – 20 ng/ml
Sensitivity< 0.188 ng/ml

Applications

This ELISA kit is used for quantitative measurement of caspase-3 in rat serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Easy, convenient and time-saving method to measure the level of caspase-3 in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
  • Detection Range: 0.313 – 20 ng/ml
  • Sensitivity: < 0.188 ng/ml
  • Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
  • No significant cross-reactivity or interference was observed.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life12 Months

Product Details

96Trace CATE4592-100
Supplier CATTRC-00172
Analyte / TargetCaspase-3 (CASP3)
CategoryCancer Biomarker ELISA Kits