TRC-00137 | Beta-2 Microglobulin (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the quantitative measurement of Beta-2 microglobulin in human serum, plasma, tissue lysate, and other biological fluids
Beta-2 microglobulin (B2M) is a low MW protein (11.8 kDa) that is a component of major histocompatibility complex (MHC) Class I molecule. It is expressed on the surface of all nucleated cells (excluding red blood cells). One of the functions of this protein is to present antigens to cytotoxic T-cells and NK cells. It is produced at a constant rate and is filtered from the blood by the kidneys. Excess concentration of B2M in serum correlates with several pathological conditions such as autoimmune disease, renal disease, inflammation, and cancer. 96Trace’s Beta-2 Microglobulin (Human) ELISA kit is designed to quantitatively measure the amount of B2M in human serum, plasma, and other biological fluids. The kit is based on the Sandwich ELISA principle. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of B2M in the sample or standard.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma,Serum,Tissue lysate,Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 1.563 - 100 ng/ml,Recovery range: 85 - 105% for normal human serum and plasma samples |
| Sensitivity | 0.938 ng/ml,Assay Precision: Intra-Assay CV < |
Applications
This Sandwich ELISA kit is designed to quantitatively measure amount of Beta-2 microglobulin in human serum, plasma, tissue lysates and other biological fluids
Features & Benefits
Sensitivity: 0.938 ng/ml,Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%,This Sandwich ELISA is highly sensitive and highly specific for the detection of B2M in human samples. There is no significant cross-reactivity or interference between B2M and analogues,Detection range: 1.563 - 100 ng/ml,Recovery range: 85 - 105% for normal human serum and plasma samples
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4879-100 |
| Supplier CAT | TRC-00137 |
| Analyte / Target | Beta-2 Microglobulin |
| Category | Cancer Biomarker ELISA Kits |
| Alternate Name | β2M, IMD43, Beta2 Microglobulin, B2M, β2-Microglobulin, Thymotaxin, Beta-2 Microglobulin |