TRC-00130 | BAFF (Human) ELISA Kit | 96 Well
Product Overview
A rapid sandwich ELISA kit for the quantitative measurement of BAFF in human serum, plasma, cell culture supernatnat and other biological fluids
B-Cell Activating Factor (BAFF) is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. 96Trace’s BAFF ELISA kit is a sandwich ELISA assay for measuring the quantity of human BAFF in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with fewer incubation and wash steps respectively, resulting in less errors and more consistent results.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Human serum, plasma, tissue homogenates and other biological fluids. |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15 - 1000 pg/ml |
| Sensitivity | < 4.5 pg/ml |
| Assay Time | 90 minutes |
Applications
This ELISA kit is used for quantitative measurement of BAFF in human serum, plasma, cell culture supernatant and other biological fluids.
Features & Benefits
- Easy, convenient and time-saving method to measure the level of BAFF in human serum, plasma, culture supernatants and other biological fluids.
- The entire assay can be performed within 90 minutes with with fewer incubation and wash step respectively, resulting in less errors and more consistent results.
- Detection Range: 15 - 1000 pg/ml
- Sensitivity: < 4.5 pg/ml
- Assay Precision: Intra-Assay: CV < 10%; Inter-Assay: CV < 12% (CV (%) = SD/mean X 100)
- Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | K4423-100 |
| Supplier CAT | TRC-00130 |
| Analyte / Target | BAFF |
| Category | Cancer Biomarker ELISA Kits |