TRC-00441 | IFN-gamma (Human) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for the quantitative measurement of IFN-gamma in Plasma,Serum,Other biological fluids.
IFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. 96Trace’s IFN-gamma (Human) ELISA kit is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-gamma. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IFN-gamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IFN-gamma bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IFN-gamma. The concentration of Human IFN-gamma in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma,Serum,Other biological fluids |
| Detection Method | Sandwich ELISA, Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.63-1000 pg/mL,Precision: Coefficient of variation is < |
| Sensitivity | 9.38 pg/mL |
Applications
This ELISA kit is used for quantitative measurement of IFN-gamma in Plasma,Serum,Other biological fluids.
Features & Benefits
- Designed for quantitative measurement of IFN-gamma.
- Assay format: Sandwich ELISA.
- Sample type: Plasma,Serum,Other biological fluids.
- Detection method: Sandwich ELISA, Absorbance (450 nm).
- Detection Range: 15.63-1000 pg/mL,Precision: Coefficient of variation is <.
- Sensitivity: 9.38 pg/mL.
- Kit size: 96 Well.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00441 |
| Analyte / Target | IFN-gamma |
| Alternate Name | IFNG, IFG, IFI, Type II Interferon |